2015

Jama'ayah Mohamed Zahidi, Tay Bee Yong, Rohaidah Hashim, Azura Mohd Noor, Siti Hawa Hamzah and Norazah Ahmad (2015). Identification of Brucella spp. Isolated from human brucellosis in Malaysia using High-melt resolution (HRM) analysis. Diagnostic Microbiology and Infectious Disease 81(4): 227-233

Molecular approaches have been investigated to overcome difficulties in identification and differentiation of Brucella spp. using conventional phenotypic methods. In this study, high-resolution melt (HRM) analysis was used for rapid identification and differentiation of members of Brucella genus. A total of 41 Brucella spp. isolates from human brucellosis were subjected to HRM analysis using 4 sets of primers, which identified 40 isolates as Brucella melitensis and 1 as Brucella canis. The technique utilized low DNA concentration and was highly reproducible. The assay is shown to be a useful diagnostic tool, which can rapidly differentiate Brucella up to species level.